Cyclic nucleotide metabolism coupled to cytodifferentiation of Blastocladiella emersonii.
نویسندگان
چکیده
Cyclic GMP and cyclic AMP levels during growth and differentiation of B. emersonii were measured by use of chemical, biochemical, and immunologic assays. Of particular interest was the finding that net synthesis of cyclic GMP occurred during a single stage of its life cycle, sporulation, when intracellular levels increased 50- to 100-fold in a process requiring protein and RNA synthesis.
منابع مشابه
Induction of cyclic AMP phosphodiesterase in Blastocladiella emersonii and its relation to cyclic AMP metabolism.
Extracts of vegetative cells of Blastocladiella emersonii contain 5% or less of the cyclic AMP phosphodiesterase activity in zoospore extracts. This difference in activity could be accounted for entirely by an increase in the differential rate of phosphodiesterase synthesis during sporulation, beginning after a lag period of about 60 min and extending for at least an additional 90 min into the ...
متن کاملChanges in cGMP phosphodiesterase levels during growth and differentiation in Blastocladiella emersonii.
Blastocladiella emersonii, a unicellular phycomycete represents a useful organism for experimental studies on the regulatory role of cyclic nucleotides (see ref [ 1 ] for a recent review of the cell cycle). Thus, the levels of CAMP** and cGMP** as well as enzyme activities concerning their metabolism were found to change markedly during the life cycle [2-51. In all living systems so far studied...
متن کاملCharacterization and submitochondrial localization of the alpha subunit of the mitochondrial processing peptidase from the aquatic fungus Blastocladiella emersonii.
In an effort to investigate the molecular mechanisms responsible for the drastic morphological changes the mitochondria go through during the life cycle of the aquatic fungus Blastocladiella emersonii, the gene encoding the alpha subunit of the mitochondrial processing peptidase (alpha-MPP) was isolated. Nucleotide sequence analysis revealed that the predicted alpha-MPP polypeptide comprises 47...
متن کاملCharacterization and Submitochondrial Localization of the a Subunit of the Mitochondrial Processing Peptidase from the Aquatic Fungus Blastocladiella emersonii
In an effort to investigate the molecular mechanisms responsible for the drastic morphological changes the mitochondria go through during the life cycle of the aquatic fungus Blastocladiella emersonii, the gene encoding the a subunit of the mitochondrial processing peptidase (a-MPP) was isolated. Nucleotide sequence analysis revealed that the predicted a-MPP polypeptide comprises 474 amino acid...
متن کاملFructose 2,6-bisphosphate and carbohydrate metabolism during the life cycle of the aquatic fungus Blastocladiella emersonii.
Removal of the growth medium and resuspension of Blastocladiella emersonii vegetative cells in a sporulation medium resulted in an abrupt fall of fructose 2,6-bisphosphate concentration to about 2% of its initial value within 10 min. The concentrations of hexose 6-phosphate and of fructose 1,6-bisphosphate also decreased by, respectively, three and tenfold over the same period. All these values...
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 72 2 شماره
صفحات -
تاریخ انتشار 1975